Measurement of the anomalous phase velocity of ballistic light in a random medium by use of a novel interferometer

Ballistic light, i.e., radiation that propagates undeflected through a turbid medium, undergoes a small change in phase velocity and exhibits unusual dispersion because of its wave nature. We use a novel highly sensitive differential phase optical interferometer to study these previously unmeasurable phenomena. We find that ballistic propagation can be classified into three regimes based on the wavelength-to-size ratio. In the regime in which the scatterer size is comparable with the wavelength, there is an anomalous phase-velocity increase as a result of adding scatterers of higher refractive index. We also observe an anomaly in the relative phase velocity, where red light is slowed more than blue light even though the added scatterers are made of material with normal dispersion

Fourier-domain low-coherence interferometry for light-scattering spectroscopy

We present a novel method for obtaining depth-resolved spectra for determining scatterer size through elastic- scattering properties. Depth resolution is achieved with a white-light source in a Michelson interferometer with the mixed signal and reference fields dispersed by a spectrograph. The spectrum is Fourier transformed to yield the axial spatial cross correlation between the signal and reference fields with near 1 m m depth resolution. Spectral information is obtained by windowing to yield the scattering amplitude as a function of wave number. The technique is demonstrated by determination of the size of polystyrene microspheres in a subsurface layer with subwavelength accuracy. Application of the technique to probing the size of cell nuclei in living epithelial tissues is discussed

Measurement of angular distributions by use of low-coherence interferometry for light-scattering spectroscopy

We present a novel interferometer for measuring angular distributions of backscattered light. The new system exploits a low-coherence source in a modified Michelson interferometer to provide depth resolution, as in optical coherence tomography, but includes an imaging system that permits the angle of the reference field to be varied in the detector plane by simple translation of an optical element. We employ this system to examine the angular distribution of light scattered by polystyrene microspheres. The measured data indicate that size information can be recovered from angular-scattering distributions and that the coherence length of the source influences the applicability of Mie theory

Phase-dispersion optical tomography

We report on phase-dispersion optical tomography, a new imaging technique based on phase measurements using low-coherence interferometry. The technique simultaneously probes the target with fundamental and second-harmonic light and interferometrically measures the relative phase shift of the backscattered light fields. This phase change can arise either from reflection at an interface within a sample or from bulk refraction. We show that this highly sensitive 5 phase technique can complement optical coherence tomography, which measures electric field amplitude, by revealing otherwise undetectable dispersive variations in the sample

Determination of particle size by using the angular distribution of backscattered light as measured with low-coherence interferometry

We employ a novel interferometer to measure the angular distribution of light backscattered by a turbid medium. Through comparison of the measured data with the predictions of Mie theory, we are able to determine the size of the scatterers comprising the medium with subwavelength precision. As the technique is based on low-coherence interferometry, we are able to examine the evolution of the angular distribution of scattered light as it propagates into the medium. The effects of multiple scattering as a function of penetration depth in the medium are analyzed. We also present various considerations for extending this technique to determining structural information in biological tissues, such as the effects of a distribution of particle sizes and the need to average out speckle contributions

Phase-referenced interferometer with subwavelength and subhertz sensitivity applied to the study of cell membrane dynamics

We report a highly sensitive means of measuring cellular dynamics with a novel interferometer that can measure motional phase changes. The system is based on a modified Michelson interferometer with a composite laser beam of 1550-nm low-coherence light and 775-nm CW light. The sample is prepared on a coverslip that is highly reflective at 775nm. By referencing the heterodyne phase of the 1550-nm light reflected from the sample to that of the 775-nm light reflected from the coverslip, small motions in the sample are detected, and motional artifacts from vibrations in the interferometer are completely eliminated. We demonstrate that the system is sensitive to motions as small as 3.6nm and velocities as small as 1nm/s. Using the instrument, we study transient volume changes of a few (approximately three) cells in a monolayer immersed in weakly hypotonic and hypertonic solutions

Equine Assisted Therapy and Changes in Gait for a Young Adult Female with Down Syndrome

The purpose of this study was to examine the effects of equine assisted therapy on selected gait parameters in a person with Down syndrome. One female participant with Down syndrome completed two therapeutic horseback riding programs, each consisting of six riding sessions. Specific gait characteristics were analyzed with a trend analysis of the data by examining the means of the different variables. The trend analysis revealed a difference in stride length as well as hip and knee angle. These results indicate that over the course of the two therapeutic horseback riding programs, changes in gait occurred. Therefore, therapeutic horseback riding may have the potential to benefit gait characteristics and stability in young adult females with Down syndrome; however, further research is warranted

Interferometric phase-dispersion microscopy

We describe a new scanning microscopy technique, phase-dispersion microscopy (PDM). The technique is based on measuring the phase difference between the fundamental and the second-harmonic light in a novel interferometer. PDM is highly sensitive to subtle refractive-index differences that are due to dispersion (differential optical path sensitivity, 5 nm). We apply PDM to measure minute amounts of DNA in solution and to study biological tissue sections. We demonstrate that PDM performs better than conventional phase-contrast microscopy in imaging dispersive and weakly scattering samples

Angular light scattering studies using low-coherence interferometry

A modified Michelson interferometer is used to measure path- length resolved angular distributions of light backscattered by turbid media. The path length resolution is obtained by exploiting the coherence properties of a broadband source. The angular distribution is mapped out using a simple optical system to scan the angle at which the reference field intersects the detector plane. Angular scattering distributions can be compared to Mie theory to determine the size and refractive index of spherical scatterers. Initial studies utilizing this system demonstrate the potential of low coherence interferometry for obtaining structural information using angular distributions